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Image Search Results
Journal: bioRxiv
Article Title: Endothelial Cept1 Promotes Post-Ischemic Angiogenesis in a Pparα -Dependent Fashion
doi: 10.1101/2025.03.11.642511
Figure Lengend Snippet: A) Western blotting demonstrates that CEPT1 content is increased in ECs isolated from Cept1 fl/fl Cre + mice. Representative blots of VEGF2R, PPAR α , ACOX1, p-Akt, t-Akt and p-eNOS, t-eNOS are also provided. B) Quantitative representation of protein content relative to GAPDH as a loading control (n=3). C) RT-PCR analysis demonstrates Cept1 is overexpressed in ECs isolated from Cept1 fl/fl Cre + mice (n=3). Gene expression of Pparα, Acox1 , Vegfa, and Vegf2r is also provided (n=3). D) Western blotting demonstrates that CEPT1 content is increased in HUVECs transduced with Cept1 lentiviral particles (MOI 5). Representative blots of VEGF2R, PPAR α , ACOX1, p-AKT, t-AKT and p-eNOS, t-eNOS are also provided. E) Quantitative representation of protein content relative to GAPDH as a loading control (n=3). F) RT-PCR analysis demonstrates Cept1 is overexpressed in transduced ECs (n=3). Gene expression of Pparα, Acox1 , Vegfa, and Vegf2r is also provided (n=3). Data are presented as mean ± SEM. * p<0.05; ** p<0.01; *** p<0.001. p values were from 1-way ANOVA and unpaired Student t -test.
Article Snippet: Proteins for western blot and immunostaining were detected with rabbit anti-CEPT1 (bs-12284R; Bioss USA),
Techniques: Western Blot, Isolation, Control, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Transduction
Journal: bioRxiv
Article Title: Endothelial Cept1 Promotes Post-Ischemic Angiogenesis in a Pparα -Dependent Fashion
doi: 10.1101/2025.03.11.642511
Figure Lengend Snippet: A-B) HUVECs were transfected with siRNA targeting Pparα or siRNA control (si-Ct) for 72 h, and the expression of Pparα was confirmed through RT-PCR. Relative gene expression of Cept1 and Pparα was performed using RT-PCR (n=3). C-D) Representative cell migration assay images at t= 0, 6, and 16h were taken from HUVEC Cept1 cDNA, Cept1 cDNA + si Pparα , and si Pparα . A quantitative graphical representation of cell migration was shown (n=3). E-F) Representative images and quantitative analysis of tubule formation of HUVEC with different conditions after 6h of incubation of Matrigel (n=3). G-H) EC from Cept1 fl/fl Cre + were treated with GW6471 (10uM), ZM323881 (80nM), LY294002 (30uM), and L- NAME (0.5uM) and cell migration at t = 0, 6, and 16h was assessed and quantified, relative to EC from Cept1 fl/fl Cre + and control (n=3). I-J) HUVEC were treated with GW6471 (10uM), ZM323881 (80nM), LY294002 (30uM), and L- NAME (0.5uM) and cell migration at t = 0, 6, and 16h was assessed and quantified, relative to control (n=3). Data are presented as mean ± SEM. * p<0.05; ** p<0.01. P values were from 1-way ANOVA and unpaired Student t -test.
Article Snippet: Proteins for western blot and immunostaining were detected with rabbit anti-CEPT1 (bs-12284R; Bioss USA),
Techniques: Transfection, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Cell Migration Assay, Migration, Incubation